advantages and disadvantages of the serial dilution agar plate technique

Preparing a series of calibration standards by this method reduces the amount of required time. What is the advantage of performing a serial dilution instead of a single dilution?Įasier and Faster Preparation of Calibration Standards The errors introduced with each successive dilution drops proportionately with the solution concentration. Essential to direct dilution is the ability to accurately transfer extremely small volumes of stock solution, which is generally not possible with pipets. This figure shows only the first four concentrations via direct dilution. The direct dilution method uses far less sample than the serial dilution method. Similarly one may ask, is direct or serial dilution more accurate? Candida krusei) were submitted to serial dilution and plating according to methods of track-dilution (TDM), drop plate (DPM) and the conventional spread. Serial dilutions are used to accurately create highly diluted solutions as well as solutions for experiments resulting in concentration curves with a logarithmic scale. Conditions can be adjusted as the experiment progresses (e.g., drug concentrations increased as drug resistance improves).įurthermore, what is serial dilution and why is it used? Serial dilution is the stepwise dilution of a substance in solution. Serial dilution has many advantages: the materials necessary are typically already present in the lab and require no special engineering. In this way, what are the advantages of serial dilution?

Advantages and disadvantages of the serial dilution agar plate technique